ranges of dna concentration in plant
Estimation of nuclear DNA content in plants using flow …
Estimation of DNA quantity (C-value) 3 in absolute units (DNA picograms and number of base pairs) has led to the discovery of more than 2,000-fold variation in the …
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Spectrophotometric measurement of DNA concentration
The concentration of DNA and RNA should be determined by measuring the absorbance at 260 nm (A 260) in a spectrophotometer. For accuracy, absorbance readings at 260 nm should fall between 0.15 and 1.0. Pure DNA has an A 260 /A 280 ratio of 1.8–2.0 in 10 mM Tris·Cl, pH 8.5. Strong absorbance at A 280 resulting in a low A 260 /A 280 ratio ...
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Plant Tissue Phosphorus Sufficiency Ranges
While soil testing is performed to predict the nutrient availability in soils, plant tissue analysis provides information on the nutrients taken up by the plants. The phosphorus concentration in plant tissue might be in deficiency range, sufficiency range, or excess range.
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A Practical Guide to Analyzing Nucleic Acid …
Manufacturer's specifications for nucleic acid detection is a lower detection limit of 2 ng/μl and reproducibility of 2 ng/μl for the concentration range 2–100 ng/μl and 2% for values …
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Quantitation of DNA and RNA
a 40 μg/mL solution of RNA. Contamination of nucleic acid solutions makes spectrophotometric quantitation inaccurate. Calculate the OD 260 /OD 280 ratio for an indication of nucleic acid purity. Pure DNA has an OD 260 /OD 280 ratio of ~1.8; pure RNA has an OD 260 /OD 280 ratio of ~2.0. Low ratios could be caused by protein or phenol …
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Quantification of DNA
DNA concentration can be determined by measuring the absorbance at 260 nm (A 260) in a spectrophotometer using a quartz cuvette. For greatest accuracy, readings should be …
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A Comprehensive High-Quality DNA and RNA Extraction …
The method presented here yielded a high quantity and concentration of DNA from 1 g of plant tissue for other plants, including capsicum ... Recommendations established here could prove adaptable to extract high-quality nucleic acids from tissues from a range of difficult plant species. Acknowledgments. The authors wish to …
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A Practical Guide to Analyzing Nucleic Acid …
for the concentration range 2–100 ng/µl and 2% for values >100 ng/µl. In buffered solutions, pure dsDNA has slightly higher A 260 /A 230 ... Assessment of DNA concentration and purity by MVS A NIH3T3 gDNA stock in TE buffer was diluted with TE buffer to obtain the indicated DNA dilutions. Concentration values (OD
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Comparison of three common DNA concentration
There are three methods commonly used to determine DNA concentration: ultraviolet (UV) absorbance, fluorescence, and diphenylamine reaction. Currently, the most common technique to determine DNA concentration is the measurement of absorbance of UV light at 260 nm [5]. The principle of the UV absorbance method is that nucleic acids …
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Efficient genomic DNA extraction protocol from medicinal …
RAPD-PCR reaction parameters such as DNA concentration (100ng), Primer concentration (2 μM), Dream Taq polymerase (2 U), annealing temperature (29°C) and number of cycles for amplification of DNA has been optimized. ... which ranges from (120 bp -2500 bp). ... Murray MG, Thompson WF. Rapid isolation of high molecular …
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Eleven Golden Rules of Quantitative RT-PCR
The following are 11 golden rules of qRT-PCR that, when observed, should ensure reproducible and accurate measurements of transcript abundance in plant and other cells. These rules are for relative quantification of RNA using two-step RT-PCR (where the product of a single RT reaction is used as template in multiple PCR reactions), SYBR Green to ...
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Concentrated DNA in PCR reaction? | ResearchGate
All Answers (10) Optimum plant DNA concentration used in PCR amplification is 20 ng/µl DNA. Higher concentration of DNA amy either result in non-specific amplicon formation. On the same side ...
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The roles of bacterial and host plant factors in …
The genetic transformation of plants mediated by Agrobacterium tumefaciens represents an essential tool for both fundamental and applied research in plant biology. For a successful infection, culminating in the integration of its transferred DNA (T-DNA) into the host genome, Agrobacterium relies on multiple interactions with host-plant factors. . Extensive studies …
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Physiological and molecular advances in magnesium nutrition of plants
Plants need a certain range of Mg concentration for their growth. Therefore, plants have to develop a variety of ways to maintain Mg homeostasis to adapt to environmental changes. Firstly, root organ shows quick responses to Mg depletion by boosting the uptake of Mg (Wang et al. 2020; Ogura et al. 2018; Tanoi et al. 2014).
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Determination of phosphorus compounds in plant tissues: …
Independent of the mentioned limitations and the different needs of individual plant species, the phosphorus concentration in plants ranges from 0.05 to 0.5% of plant dry weight . This element occurs in plants either as the free inorganic orthophosphate group (Pi) or as organophosphorus compounds . Pi existing in plant cells is divided into two ...
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Addgene: DNA Quantification
If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record the concentration and purity. Note: Purity is measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample. Note: Keep in mind that despite the accuracy of the ...
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A universal protocol for high-quality DNA and RNA isolation …
These steps resulted in isolating a high concentration of DNA and RNA from plant tissues, followed by purification of nucleic acids using solution-based (Fig 1, Steps 5S-7S) and column-based (Fig 1, Steps 5C-8C) approaches. Depending on the purpose of the research, either approach can be used for nucleic acid purification.
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Characterisation of insect and plant origins using DNA extracted …
A DNA-based tool was validated that potentially enables the characterisation of both plant and insect of origin of small (approximately 1 ml) samples of bee honey. Using this method, mitochondrial, nuclear and chloroplast DNA (mtDNA, nuDNA, cpDNA) markers were successfully extracted, PCR amplified, and sequenced from a range of honeys, and …
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(PDF) DNA QUANTIFICATION
DNA QUANTIFICATION. Karen P. Pachchigar 1, Akhshay Khunt 2 and Bhilocha Hetal 3. Background: Quantification and assessment of DNA/RNA and Protein purity and concentration, is. first entry step in ...
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DNA extract characterization process for microbial detection …
The DNA concentration measurements made using the Nanodrop were ... steps may have resulted in loss of genomic DNA. A range of different size beads are used in the bead beating step of precipB which may aide in lysis of different cell types resulting in ... based analysis of fungal and bacterial DNA. Plant Mol Biol Rep. 2004; 22:71–81 ...
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Interpreting Nanodrop (Spectrophotometric) Results
Where A=absorbance, ԑ=extinction coefficient, c=concentration and l=path length. The Beer‐Lambert law draws a direct correlation between absorbance and concentration. ... Samples outside of this range should be dried‐down or diluted to produce more accurate ... DNA, A260/280 ratios should be somewhere around 2.1 and 1.8, respectively. A ...
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Comparison of Methods for DNA and RNA Purification …
Figure 1. Analyses of DNA eluates isolated from plant leaves using two nucleic acid chemistries. Panel A. DNA yield from plant leaf tissues. DNA quantitation was performed using 1µl of purified plant DNA with the QuantiFluor® dsDNA System on the Quantus™ Fluorometer. Panel B. Absorbance ratios of DNA from plant leaf tissue.
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A rapid procedure for the isolation of C0t-1 DNA from …
Once all the calculations have been made, the DNA is denatured by placing the 15-mL tube in a 95°C water bath for 10 min. 8. Remove the tube, cool it by swirling in ice water for 10 s, and place it in the 65°C water bath. Start timing the reannealing period for the calculated Cot- 1 time. Sl nuclease digestion.
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Comparison of three common DNA concentration measurement methods
PicoGreen. Proteins. RNA, Transfer. Diphenylamine. Accurate measurement of DNA concentration is important for DNA-based biological applications. DNA concentration is usually determined by the ultraviolet (UV) absorption, fluorescence staining, and diphenylamine reaction methods. However, the best method for quality …
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Methods for Determining DNA Yield and Concentration
To ensure the numbers are useful, the A 260 reading should be within the instrument's linear range (generally 0.1–1.0). DNA concentration is estimated by measuring the absorbance at 260nm, adjusting the A 260 measurement for turbidity (measured by absorbance at 320nm), multiplying by the dilution factor, and using the …
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DNA Quantitation: How to Measure DNA Concentration
This step corrects for any absorbance due to the buffer. DNA Measurement: Measure the absorbance of your diluted DNA sample at 260 nm to determine DNA concentration and at 280 nm to assess purity. Pure DNA has an A260/A280 ratio of ~1.8. Calculate Concentration: Use the formula where 50 µg/mL is the extinction coefficient of DNA, to …
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DNA analysis using analytical gels
Analysing DNA. DNA analysis using analytical gels. Gels allow separation and identification of nucleic acids based on charge migration. Migration of nucleic acid molecules in an electric field is determined by size and conformation, allowing nucleic fragments of different sizes to be separated. However, the relationship between the fragment ...
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DNA CONCENTRATION MEASUREMENT AT 260 nm USING …
DNA CONCENTRATION MEASUREMENT AT 260 nm USING PHOTOPETTE® BIO
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DNA concentration and quality in plant …
DNA concentrations and purity ratios were variable; the highest concentration was obtained with the MN kit (170.45 ng/μl), but with deficiencies in purity (0.32 of A260/A230, 0.34 of A260/A280).
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How to determine the concentration and purity of a DNA …
The ratio of absorbance at 260 and 280nm is used to assess the purity of DNA and RNA. A ratio of ~1.8 is generally accepted as "pure" for DNA; a ratio of ~2.0 is generally accepted as "pure" for RNA. If the ratio is appreciably lower in either case, it may indicate the presence of protein, phenol or other contaminants that absorb ...
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